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    Greiner Bio thincert cell culture inserts greiner bio one
    Thincert Cell Culture Inserts Greiner Bio One, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thincert+cell+culture+inserts+greiner+bio+one/Thincert+Cell+Culture+Insert+For+6+Well+Plates/pm41767677-91-31-35
    Average 96 stars, based on 675 article reviews
    thincert cell culture inserts greiner bio one - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human iPSC line: ZIPi013-B ZIP gGmbH; Tandon et al. 62 RRID: CVCL_UF44 Human iPSC line: HHUUKDi009-A HHU Dü sseldorf; Lorenz et al. 63 RRID: CVCL_B3T9 Human melanoma cell line: A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 Human melanoma cell line: MelDCC3 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC8 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC11 University of Regensburg/ Fraunhofer ITEM-R N/A Recombinant DNA pRRL-CMV-GFP-puro Swiss Institute of Cancer Research N/A LeGO-T2 University Medical Center Hamburg- Eppendorf Addgene, Cat#27342; RRID: Addgene_27342 Software and algorithm Kaleido software Revvity N/A VisiSens scientifical software PreSens https://www.presens.de/de/produkte/ detail/visisens-scientifical-software ImageJ 1.54 NIH https://imagej.net; RRID: SCR_003070 Imaris 10.0.0 Oxford Instruments http://www.bitplane.com/imaris/imaris; RRID: SCR_007370 Harmony Revvity https://www.perkinelmer.com/product/ harmony-5-1-office-hh17000012; RRID: SCR_023543 Patch Master V2x80 HEKA Elektronik https://www.heka.com/downloads/ downloads_main.html#down_patchmaster Origin Pro OriginLab http://www.originlab.com/index. aspx?go=PRODUCTS/Origin; RRID: SCR_014212 STAR v.2.4 Dobin et al. 64 http://code.google.com/p/rna-star/; RRID: SCR_004463 featureCounts v.1.5.1 Liao et al. 65 http://bioinf.wehi.edu.au/featureCounts/; RRID: SCR_012919 DESeq2 v.3.12 Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID: SCR_015687 biomaRt v.4.0 Durinck et al. 67 https://bioconductor.org/packages/ biomaRt/; RRID: SCR_019214 clusterProfiler Yu et al. 68 http://bioconductor.org/packages/release/ bioc/html/clusterProfiler.html; RRID: SCR_016884 STRING Global Biodata Coalition and ELIXIR http://string.embl.de/; RRID: SCR_005223 FlowJo BD Biosciences https://www.flowjo.com/solutions/flowjo; RRID: SCR_008520 Analyst 1.7.2 Sciex https://sciex.com/products/software/ analyst-software GraphPad Prism 10 GraphPad http://www.graphpad.com/; RRID: SCR_002798 Other 6-well plates Greiner Bio-One Cat# 657-160 AggreWell 800 STEMCELL Technologies Cat# 34815 96-well ultra-low attachment plates Corning Cat# 7007 (Continued on next page) e4 Cell Reports Methods 5, 101236, December 15, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Nunc 6-well plates Thermo Scientific Cat# 140675 ThinCert cell culture inserts Greiner Bio-One Cat# 662-641 Corning mini bioreactor Sigma-Aldrich Cat# CLS431720 24-well plates Greiner Bio-One Cat# 662160 35 mm Petri dish Thermo Scientific Cat# 130180 Echo Qualified 384-well plates Labcyte Cat# 001-16128 384-well plates Greiner Bio-One Cat# 781091 96-well cell culture microplate, CELLSTAR, black Greiner Bio-One Cat# 655090 96-well cell culture microplate, CELLSTAR, white Greiner Bio-One Cat# 655083 Breathseal sealer Greiner Bio-One Cat# 676050 Multi-channel micropipettes Brand N/A Cell culture warming plate Labotect N/A EnSight multimode plate reader Revvity N/A Oxygen-sensor foils PreSens Cat# SF-RPSu4 VisiSens TD PreSens N/A CS-Mount Lens ArduCam Cat# CS2325ZM01 Silicon glue RS Components N/A Muvi SPIM Bruker N/A ASP300S tissue processor Leica biosystems N/A Antigen Retriever 2100 Aptum N/A Operetta CLS High Content Imaging System Revvity N/A Ventana BenchMark XT autostainer Ventana N/A Zeiss Axioscope 5 microscope Zeiss N/A Axiocam 208 color camera Zeiss N/A NanoDrop 1000 spectrophotometer Thermo Fisher Scientific N/A QuantStudio 6 Flex Real-Time-PCRSystem Applied Biosystems N/A EPC 10 USB System with Red Star Headstage Warner Instruments Cat# 89-5273 Eclipse FN1 microscope Nikon N/A LYNX microscopy tissue processor Reichert-Jung N/A Side-mounted 2kx2k camera TRS TRO ̈ NDLE Restlichtverstä rkersysteme N/A LEO 912AB electron-microscope ZEISS N/A Ultracut S Microtome Reichert-Jung N/A Diamond knives DiATOME N/A Autosamdri-815 Tousimi N/A Goldpalladium Leica Microsystems Cat# EM ACE 200 Crossbeam 550 Zeiss N/A FACSymphony A3 BD Biosciences N/A Gallios 10C 3L Flow Cytometer Beckmann Coulter N/A Echo 550 liquid handler Labcyte N/A MultiDrop Thermo Fisher Scientific N/A Standard Tube Cassettes Steinle Labtechnology N/A EnVision Multimode Plate Reader Revvity N/A cellZscope nanoAnalytics N/A Infinite M1000 Pro TECAN N/A MTS4 microtiter shaker IKA N/A (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e5 OPEN ACCESS ..

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma.
    Article Snippet: .. SDS lysis buffer {2% SDS, 50 mM Tris–HCl (pH 6.8), and 1 mM EDTA (pH 8.0)} was added to cultured cells after washing them twice Reagent Product name Supplier Catalog number ThinCert Cell Culture Inserts Greiner Bio-One 657630 0.2 μm pore filter Sartorius S7597FXOSK Dead Cell Removal Kit Miltenyi Biotec, Bergisch Gladbach, Germany 130-090-101 Chromium Next GEM Single Cell 3′ Kit v3.1, 4 rxns 10x Genomics, CA, USA PN-1000269 Chromium Next GEM Chip G Single Cell Kit, 16 rxns 10x Genomics PN-1000127 Dual Index Kit TT Set A, 96 rxns 10x Genomics PN-1000215 High Sensitivity DNA Kit Agilent Technology 5067-4727 Tissue-Tek® O.C.T. .. Compound Sakura Finetek Japan Co., Ltd., Tokyo, Japan 4583 13C5 L-glutamine Fujifilm Wako Pure Chemical Industries, Osaka, Japan CLM-1822-H-0.1 erythritol Tokyo Chemical Industry Co., Ltd., Tokyo, Japan E0021 Ultracentrifugation for 5 kDa cut-off filter Human Metabolome Technology, Tsuruoka, Japan UFC3LCCNB_HMT 96 well cell culture plates Greiner Bio-One 655180 Cell Counting Kit-8 Dojindo 343-07623 Eukitt ORSAtec, Bobingen, Germany 6.00.01.0001.06.01.EN BRANSON Sonifier 450 Branson Ultrasonics Corporation, CT, USA Instruments Product Name Supplier Catalog number Image Quant LAS 4000 mini luminescent image analyzer Cytiva, MA, USA NanoZoomer 2.0-RS Hamamatsu Photonics, Hamamatsu, Japan CellDrop BF DeNovix, DE, USA Seahorse XFp Analyzer Agilent Technology EVOS FL Thermo Fisher Scientific Qubit Invitrogen, MA, USA TapeStation Agilent Technology StepOne Real-time PCR system Thermo Fisher Scientific BX-41 Olympus, Tokyo, Japan Chromium controller 10x Genomics MTP-320 Corona Electric Co., Ltd., Ibaraki, Japan Tissue-Tek VIP5 Jr. Sakura Finetek Japan Co., Ltd TABLE 2 (Continued) Frontiers in Veterinary Science 06 frontiersin.org with ice-cold PBS.

    Imaging:

    Article Title: Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human iPSC line: ZIPi013-B ZIP gGmbH; Tandon et al. 62 RRID: CVCL_UF44 Human iPSC line: HHUUKDi009-A HHU Dü sseldorf; Lorenz et al. 63 RRID: CVCL_B3T9 Human melanoma cell line: A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 Human melanoma cell line: MelDCC3 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC8 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC11 University of Regensburg/ Fraunhofer ITEM-R N/A Recombinant DNA pRRL-CMV-GFP-puro Swiss Institute of Cancer Research N/A LeGO-T2 University Medical Center Hamburg- Eppendorf Addgene, Cat#27342; RRID: Addgene_27342 Software and algorithm Kaleido software Revvity N/A VisiSens scientifical software PreSens https://www.presens.de/de/produkte/ detail/visisens-scientifical-software ImageJ 1.54 NIH https://imagej.net; RRID: SCR_003070 Imaris 10.0.0 Oxford Instruments http://www.bitplane.com/imaris/imaris; RRID: SCR_007370 Harmony Revvity https://www.perkinelmer.com/product/ harmony-5-1-office-hh17000012; RRID: SCR_023543 Patch Master V2x80 HEKA Elektronik https://www.heka.com/downloads/ downloads_main.html#down_patchmaster Origin Pro OriginLab http://www.originlab.com/index. aspx?go=PRODUCTS/Origin; RRID: SCR_014212 STAR v.2.4 Dobin et al. 64 http://code.google.com/p/rna-star/; RRID: SCR_004463 featureCounts v.1.5.1 Liao et al. 65 http://bioinf.wehi.edu.au/featureCounts/; RRID: SCR_012919 DESeq2 v.3.12 Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID: SCR_015687 biomaRt v.4.0 Durinck et al. 67 https://bioconductor.org/packages/ biomaRt/; RRID: SCR_019214 clusterProfiler Yu et al. 68 http://bioconductor.org/packages/release/ bioc/html/clusterProfiler.html; RRID: SCR_016884 STRING Global Biodata Coalition and ELIXIR http://string.embl.de/; RRID: SCR_005223 FlowJo BD Biosciences https://www.flowjo.com/solutions/flowjo; RRID: SCR_008520 Analyst 1.7.2 Sciex https://sciex.com/products/software/ analyst-software GraphPad Prism 10 GraphPad http://www.graphpad.com/; RRID: SCR_002798 Other 6-well plates Greiner Bio-One Cat# 657-160 AggreWell 800 STEMCELL Technologies Cat# 34815 96-well ultra-low attachment plates Corning Cat# 7007 (Continued on next page) e4 Cell Reports Methods 5, 101236, December 15, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Nunc 6-well plates Thermo Scientific Cat# 140675 ThinCert cell culture inserts Greiner Bio-One Cat# 662-641 Corning mini bioreactor Sigma-Aldrich Cat# CLS431720 24-well plates Greiner Bio-One Cat# 662160 35 mm Petri dish Thermo Scientific Cat# 130180 Echo Qualified 384-well plates Labcyte Cat# 001-16128 384-well plates Greiner Bio-One Cat# 781091 96-well cell culture microplate, CELLSTAR, black Greiner Bio-One Cat# 655090 96-well cell culture microplate, CELLSTAR, white Greiner Bio-One Cat# 655083 Breathseal sealer Greiner Bio-One Cat# 676050 Multi-channel micropipettes Brand N/A Cell culture warming plate Labotect N/A EnSight multimode plate reader Revvity N/A Oxygen-sensor foils PreSens Cat# SF-RPSu4 VisiSens TD PreSens N/A CS-Mount Lens ArduCam Cat# CS2325ZM01 Silicon glue RS Components N/A Muvi SPIM Bruker N/A ASP300S tissue processor Leica biosystems N/A Antigen Retriever 2100 Aptum N/A Operetta CLS High Content Imaging System Revvity N/A Ventana BenchMark XT autostainer Ventana N/A Zeiss Axioscope 5 microscope Zeiss N/A Axiocam 208 color camera Zeiss N/A NanoDrop 1000 spectrophotometer Thermo Fisher Scientific N/A QuantStudio 6 Flex Real-Time-PCRSystem Applied Biosystems N/A EPC 10 USB System with Red Star Headstage Warner Instruments Cat# 89-5273 Eclipse FN1 microscope Nikon N/A LYNX microscopy tissue processor Reichert-Jung N/A Side-mounted 2kx2k camera TRS TRO ̈ NDLE Restlichtverstä rkersysteme N/A LEO 912AB electron-microscope ZEISS N/A Ultracut S Microtome Reichert-Jung N/A Diamond knives DiATOME N/A Autosamdri-815 Tousimi N/A Goldpalladium Leica Microsystems Cat# EM ACE 200 Crossbeam 550 Zeiss N/A FACSymphony A3 BD Biosciences N/A Gallios 10C 3L Flow Cytometer Beckmann Coulter N/A Echo 550 liquid handler Labcyte N/A MultiDrop Thermo Fisher Scientific N/A Standard Tube Cassettes Steinle Labtechnology N/A EnVision Multimode Plate Reader Revvity N/A cellZscope nanoAnalytics N/A Infinite M1000 Pro TECAN N/A MTS4 microtiter shaker IKA N/A (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e5 OPEN ACCESS ..

    Microscopy:

    Article Title: Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human iPSC line: ZIPi013-B ZIP gGmbH; Tandon et al. 62 RRID: CVCL_UF44 Human iPSC line: HHUUKDi009-A HHU Dü sseldorf; Lorenz et al. 63 RRID: CVCL_B3T9 Human melanoma cell line: A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 Human melanoma cell line: MelDCC3 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC8 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC11 University of Regensburg/ Fraunhofer ITEM-R N/A Recombinant DNA pRRL-CMV-GFP-puro Swiss Institute of Cancer Research N/A LeGO-T2 University Medical Center Hamburg- Eppendorf Addgene, Cat#27342; RRID: Addgene_27342 Software and algorithm Kaleido software Revvity N/A VisiSens scientifical software PreSens https://www.presens.de/de/produkte/ detail/visisens-scientifical-software ImageJ 1.54 NIH https://imagej.net; RRID: SCR_003070 Imaris 10.0.0 Oxford Instruments http://www.bitplane.com/imaris/imaris; RRID: SCR_007370 Harmony Revvity https://www.perkinelmer.com/product/ harmony-5-1-office-hh17000012; RRID: SCR_023543 Patch Master V2x80 HEKA Elektronik https://www.heka.com/downloads/ downloads_main.html#down_patchmaster Origin Pro OriginLab http://www.originlab.com/index. aspx?go=PRODUCTS/Origin; RRID: SCR_014212 STAR v.2.4 Dobin et al. 64 http://code.google.com/p/rna-star/; RRID: SCR_004463 featureCounts v.1.5.1 Liao et al. 65 http://bioinf.wehi.edu.au/featureCounts/; RRID: SCR_012919 DESeq2 v.3.12 Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID: SCR_015687 biomaRt v.4.0 Durinck et al. 67 https://bioconductor.org/packages/ biomaRt/; RRID: SCR_019214 clusterProfiler Yu et al. 68 http://bioconductor.org/packages/release/ bioc/html/clusterProfiler.html; RRID: SCR_016884 STRING Global Biodata Coalition and ELIXIR http://string.embl.de/; RRID: SCR_005223 FlowJo BD Biosciences https://www.flowjo.com/solutions/flowjo; RRID: SCR_008520 Analyst 1.7.2 Sciex https://sciex.com/products/software/ analyst-software GraphPad Prism 10 GraphPad http://www.graphpad.com/; RRID: SCR_002798 Other 6-well plates Greiner Bio-One Cat# 657-160 AggreWell 800 STEMCELL Technologies Cat# 34815 96-well ultra-low attachment plates Corning Cat# 7007 (Continued on next page) e4 Cell Reports Methods 5, 101236, December 15, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Nunc 6-well plates Thermo Scientific Cat# 140675 ThinCert cell culture inserts Greiner Bio-One Cat# 662-641 Corning mini bioreactor Sigma-Aldrich Cat# CLS431720 24-well plates Greiner Bio-One Cat# 662160 35 mm Petri dish Thermo Scientific Cat# 130180 Echo Qualified 384-well plates Labcyte Cat# 001-16128 384-well plates Greiner Bio-One Cat# 781091 96-well cell culture microplate, CELLSTAR, black Greiner Bio-One Cat# 655090 96-well cell culture microplate, CELLSTAR, white Greiner Bio-One Cat# 655083 Breathseal sealer Greiner Bio-One Cat# 676050 Multi-channel micropipettes Brand N/A Cell culture warming plate Labotect N/A EnSight multimode plate reader Revvity N/A Oxygen-sensor foils PreSens Cat# SF-RPSu4 VisiSens TD PreSens N/A CS-Mount Lens ArduCam Cat# CS2325ZM01 Silicon glue RS Components N/A Muvi SPIM Bruker N/A ASP300S tissue processor Leica biosystems N/A Antigen Retriever 2100 Aptum N/A Operetta CLS High Content Imaging System Revvity N/A Ventana BenchMark XT autostainer Ventana N/A Zeiss Axioscope 5 microscope Zeiss N/A Axiocam 208 color camera Zeiss N/A NanoDrop 1000 spectrophotometer Thermo Fisher Scientific N/A QuantStudio 6 Flex Real-Time-PCRSystem Applied Biosystems N/A EPC 10 USB System with Red Star Headstage Warner Instruments Cat# 89-5273 Eclipse FN1 microscope Nikon N/A LYNX microscopy tissue processor Reichert-Jung N/A Side-mounted 2kx2k camera TRS TRO ̈ NDLE Restlichtverstä rkersysteme N/A LEO 912AB electron-microscope ZEISS N/A Ultracut S Microtome Reichert-Jung N/A Diamond knives DiATOME N/A Autosamdri-815 Tousimi N/A Goldpalladium Leica Microsystems Cat# EM ACE 200 Crossbeam 550 Zeiss N/A FACSymphony A3 BD Biosciences N/A Gallios 10C 3L Flow Cytometer Beckmann Coulter N/A Echo 550 liquid handler Labcyte N/A MultiDrop Thermo Fisher Scientific N/A Standard Tube Cassettes Steinle Labtechnology N/A EnVision Multimode Plate Reader Revvity N/A cellZscope nanoAnalytics N/A Infinite M1000 Pro TECAN N/A MTS4 microtiter shaker IKA N/A (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e5 OPEN ACCESS ..

    Spectrophotometry:

    Article Title: Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human iPSC line: ZIPi013-B ZIP gGmbH; Tandon et al. 62 RRID: CVCL_UF44 Human iPSC line: HHUUKDi009-A HHU Dü sseldorf; Lorenz et al. 63 RRID: CVCL_B3T9 Human melanoma cell line: A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 Human melanoma cell line: MelDCC3 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC8 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC11 University of Regensburg/ Fraunhofer ITEM-R N/A Recombinant DNA pRRL-CMV-GFP-puro Swiss Institute of Cancer Research N/A LeGO-T2 University Medical Center Hamburg- Eppendorf Addgene, Cat#27342; RRID: Addgene_27342 Software and algorithm Kaleido software Revvity N/A VisiSens scientifical software PreSens https://www.presens.de/de/produkte/ detail/visisens-scientifical-software ImageJ 1.54 NIH https://imagej.net; RRID: SCR_003070 Imaris 10.0.0 Oxford Instruments http://www.bitplane.com/imaris/imaris; RRID: SCR_007370 Harmony Revvity https://www.perkinelmer.com/product/ harmony-5-1-office-hh17000012; RRID: SCR_023543 Patch Master V2x80 HEKA Elektronik https://www.heka.com/downloads/ downloads_main.html#down_patchmaster Origin Pro OriginLab http://www.originlab.com/index. aspx?go=PRODUCTS/Origin; RRID: SCR_014212 STAR v.2.4 Dobin et al. 64 http://code.google.com/p/rna-star/; RRID: SCR_004463 featureCounts v.1.5.1 Liao et al. 65 http://bioinf.wehi.edu.au/featureCounts/; RRID: SCR_012919 DESeq2 v.3.12 Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID: SCR_015687 biomaRt v.4.0 Durinck et al. 67 https://bioconductor.org/packages/ biomaRt/; RRID: SCR_019214 clusterProfiler Yu et al. 68 http://bioconductor.org/packages/release/ bioc/html/clusterProfiler.html; RRID: SCR_016884 STRING Global Biodata Coalition and ELIXIR http://string.embl.de/; RRID: SCR_005223 FlowJo BD Biosciences https://www.flowjo.com/solutions/flowjo; RRID: SCR_008520 Analyst 1.7.2 Sciex https://sciex.com/products/software/ analyst-software GraphPad Prism 10 GraphPad http://www.graphpad.com/; RRID: SCR_002798 Other 6-well plates Greiner Bio-One Cat# 657-160 AggreWell 800 STEMCELL Technologies Cat# 34815 96-well ultra-low attachment plates Corning Cat# 7007 (Continued on next page) e4 Cell Reports Methods 5, 101236, December 15, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Nunc 6-well plates Thermo Scientific Cat# 140675 ThinCert cell culture inserts Greiner Bio-One Cat# 662-641 Corning mini bioreactor Sigma-Aldrich Cat# CLS431720 24-well plates Greiner Bio-One Cat# 662160 35 mm Petri dish Thermo Scientific Cat# 130180 Echo Qualified 384-well plates Labcyte Cat# 001-16128 384-well plates Greiner Bio-One Cat# 781091 96-well cell culture microplate, CELLSTAR, black Greiner Bio-One Cat# 655090 96-well cell culture microplate, CELLSTAR, white Greiner Bio-One Cat# 655083 Breathseal sealer Greiner Bio-One Cat# 676050 Multi-channel micropipettes Brand N/A Cell culture warming plate Labotect N/A EnSight multimode plate reader Revvity N/A Oxygen-sensor foils PreSens Cat# SF-RPSu4 VisiSens TD PreSens N/A CS-Mount Lens ArduCam Cat# CS2325ZM01 Silicon glue RS Components N/A Muvi SPIM Bruker N/A ASP300S tissue processor Leica biosystems N/A Antigen Retriever 2100 Aptum N/A Operetta CLS High Content Imaging System Revvity N/A Ventana BenchMark XT autostainer Ventana N/A Zeiss Axioscope 5 microscope Zeiss N/A Axiocam 208 color camera Zeiss N/A NanoDrop 1000 spectrophotometer Thermo Fisher Scientific N/A QuantStudio 6 Flex Real-Time-PCRSystem Applied Biosystems N/A EPC 10 USB System with Red Star Headstage Warner Instruments Cat# 89-5273 Eclipse FN1 microscope Nikon N/A LYNX microscopy tissue processor Reichert-Jung N/A Side-mounted 2kx2k camera TRS TRO ̈ NDLE Restlichtverstä rkersysteme N/A LEO 912AB electron-microscope ZEISS N/A Ultracut S Microtome Reichert-Jung N/A Diamond knives DiATOME N/A Autosamdri-815 Tousimi N/A Goldpalladium Leica Microsystems Cat# EM ACE 200 Crossbeam 550 Zeiss N/A FACSymphony A3 BD Biosciences N/A Gallios 10C 3L Flow Cytometer Beckmann Coulter N/A Echo 550 liquid handler Labcyte N/A MultiDrop Thermo Fisher Scientific N/A Standard Tube Cassettes Steinle Labtechnology N/A EnVision Multimode Plate Reader Revvity N/A cellZscope nanoAnalytics N/A Infinite M1000 Pro TECAN N/A MTS4 microtiter shaker IKA N/A (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e5 OPEN ACCESS ..

    Flow Cytometry:

    Article Title: Cortical organoid-derived models of the melanoma brain metastatic niche enable prioritization of cancer-targeting drugs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human iPSC line: ZIPi013-B ZIP gGmbH; Tandon et al. 62 RRID: CVCL_UF44 Human iPSC line: HHUUKDi009-A HHU Dü sseldorf; Lorenz et al. 63 RRID: CVCL_B3T9 Human melanoma cell line: A375 ATCC Cat# CRL-1619; RRID: CVCL_0132 Human melanoma cell line: MelDCC3 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC8 University of Regensburg/ Fraunhofer ITEM-R N/A Human melanoma cell line: MelDCC11 University of Regensburg/ Fraunhofer ITEM-R N/A Recombinant DNA pRRL-CMV-GFP-puro Swiss Institute of Cancer Research N/A LeGO-T2 University Medical Center Hamburg- Eppendorf Addgene, Cat#27342; RRID: Addgene_27342 Software and algorithm Kaleido software Revvity N/A VisiSens scientifical software PreSens https://www.presens.de/de/produkte/ detail/visisens-scientifical-software ImageJ 1.54 NIH https://imagej.net; RRID: SCR_003070 Imaris 10.0.0 Oxford Instruments http://www.bitplane.com/imaris/imaris; RRID: SCR_007370 Harmony Revvity https://www.perkinelmer.com/product/ harmony-5-1-office-hh17000012; RRID: SCR_023543 Patch Master V2x80 HEKA Elektronik https://www.heka.com/downloads/ downloads_main.html#down_patchmaster Origin Pro OriginLab http://www.originlab.com/index. aspx?go=PRODUCTS/Origin; RRID: SCR_014212 STAR v.2.4 Dobin et al. 64 http://code.google.com/p/rna-star/; RRID: SCR_004463 featureCounts v.1.5.1 Liao et al. 65 http://bioinf.wehi.edu.au/featureCounts/; RRID: SCR_012919 DESeq2 v.3.12 Love et al. 66 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID: SCR_015687 biomaRt v.4.0 Durinck et al. 67 https://bioconductor.org/packages/ biomaRt/; RRID: SCR_019214 clusterProfiler Yu et al. 68 http://bioconductor.org/packages/release/ bioc/html/clusterProfiler.html; RRID: SCR_016884 STRING Global Biodata Coalition and ELIXIR http://string.embl.de/; RRID: SCR_005223 FlowJo BD Biosciences https://www.flowjo.com/solutions/flowjo; RRID: SCR_008520 Analyst 1.7.2 Sciex https://sciex.com/products/software/ analyst-software GraphPad Prism 10 GraphPad http://www.graphpad.com/; RRID: SCR_002798 Other 6-well plates Greiner Bio-One Cat# 657-160 AggreWell 800 STEMCELL Technologies Cat# 34815 96-well ultra-low attachment plates Corning Cat# 7007 (Continued on next page) e4 Cell Reports Methods 5, 101236, December 15, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Nunc 6-well plates Thermo Scientific Cat# 140675 ThinCert cell culture inserts Greiner Bio-One Cat# 662-641 Corning mini bioreactor Sigma-Aldrich Cat# CLS431720 24-well plates Greiner Bio-One Cat# 662160 35 mm Petri dish Thermo Scientific Cat# 130180 Echo Qualified 384-well plates Labcyte Cat# 001-16128 384-well plates Greiner Bio-One Cat# 781091 96-well cell culture microplate, CELLSTAR, black Greiner Bio-One Cat# 655090 96-well cell culture microplate, CELLSTAR, white Greiner Bio-One Cat# 655083 Breathseal sealer Greiner Bio-One Cat# 676050 Multi-channel micropipettes Brand N/A Cell culture warming plate Labotect N/A EnSight multimode plate reader Revvity N/A Oxygen-sensor foils PreSens Cat# SF-RPSu4 VisiSens TD PreSens N/A CS-Mount Lens ArduCam Cat# CS2325ZM01 Silicon glue RS Components N/A Muvi SPIM Bruker N/A ASP300S tissue processor Leica biosystems N/A Antigen Retriever 2100 Aptum N/A Operetta CLS High Content Imaging System Revvity N/A Ventana BenchMark XT autostainer Ventana N/A Zeiss Axioscope 5 microscope Zeiss N/A Axiocam 208 color camera Zeiss N/A NanoDrop 1000 spectrophotometer Thermo Fisher Scientific N/A QuantStudio 6 Flex Real-Time-PCRSystem Applied Biosystems N/A EPC 10 USB System with Red Star Headstage Warner Instruments Cat# 89-5273 Eclipse FN1 microscope Nikon N/A LYNX microscopy tissue processor Reichert-Jung N/A Side-mounted 2kx2k camera TRS TRO ̈ NDLE Restlichtverstä rkersysteme N/A LEO 912AB electron-microscope ZEISS N/A Ultracut S Microtome Reichert-Jung N/A Diamond knives DiATOME N/A Autosamdri-815 Tousimi N/A Goldpalladium Leica Microsystems Cat# EM ACE 200 Crossbeam 550 Zeiss N/A FACSymphony A3 BD Biosciences N/A Gallios 10C 3L Flow Cytometer Beckmann Coulter N/A Echo 550 liquid handler Labcyte N/A MultiDrop Thermo Fisher Scientific N/A Standard Tube Cassettes Steinle Labtechnology N/A EnVision Multimode Plate Reader Revvity N/A cellZscope nanoAnalytics N/A Infinite M1000 Pro TECAN N/A MTS4 microtiter shaker IKA N/A (Continued on next page) Cell Reports Methods 5, 101236, December 15, 2025 e5 OPEN ACCESS ..

    Lysis:

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma.
    Article Snippet: .. SDS lysis buffer {2% SDS, 50 mM Tris–HCl (pH 6.8), and 1 mM EDTA (pH 8.0)} was added to cultured cells after washing them twice Reagent Product name Supplier Catalog number ThinCert Cell Culture Inserts Greiner Bio-One 657630 0.2 μm pore filter Sartorius S7597FXOSK Dead Cell Removal Kit Miltenyi Biotec, Bergisch Gladbach, Germany 130-090-101 Chromium Next GEM Single Cell 3′ Kit v3.1, 4 rxns 10x Genomics, CA, USA PN-1000269 Chromium Next GEM Chip G Single Cell Kit, 16 rxns 10x Genomics PN-1000127 Dual Index Kit TT Set A, 96 rxns 10x Genomics PN-1000215 High Sensitivity DNA Kit Agilent Technology 5067-4727 Tissue-Tek® O.C.T. .. Compound Sakura Finetek Japan Co., Ltd., Tokyo, Japan 4583 13C5 L-glutamine Fujifilm Wako Pure Chemical Industries, Osaka, Japan CLM-1822-H-0.1 erythritol Tokyo Chemical Industry Co., Ltd., Tokyo, Japan E0021 Ultracentrifugation for 5 kDa cut-off filter Human Metabolome Technology, Tsuruoka, Japan UFC3LCCNB_HMT 96 well cell culture plates Greiner Bio-One 655180 Cell Counting Kit-8 Dojindo 343-07623 Eukitt ORSAtec, Bobingen, Germany 6.00.01.0001.06.01.EN BRANSON Sonifier 450 Branson Ultrasonics Corporation, CT, USA Instruments Product Name Supplier Catalog number Image Quant LAS 4000 mini luminescent image analyzer Cytiva, MA, USA NanoZoomer 2.0-RS Hamamatsu Photonics, Hamamatsu, Japan CellDrop BF DeNovix, DE, USA Seahorse XFp Analyzer Agilent Technology EVOS FL Thermo Fisher Scientific Qubit Invitrogen, MA, USA TapeStation Agilent Technology StepOne Real-time PCR system Thermo Fisher Scientific BX-41 Olympus, Tokyo, Japan Chromium controller 10x Genomics MTP-320 Corona Electric Co., Ltd., Ibaraki, Japan Tissue-Tek VIP5 Jr. Sakura Finetek Japan Co., Ltd TABLE 2 (Continued) Frontiers in Veterinary Science 06 frontiersin.org with ice-cold PBS.

    Single Cell:

    Article Title: Lysine lactylation regulates ATF4-mediated stress responses under glucose starvation in canine hemangiosarcoma.
    Article Snippet: .. SDS lysis buffer {2% SDS, 50 mM Tris–HCl (pH 6.8), and 1 mM EDTA (pH 8.0)} was added to cultured cells after washing them twice Reagent Product name Supplier Catalog number ThinCert Cell Culture Inserts Greiner Bio-One 657630 0.2 μm pore filter Sartorius S7597FXOSK Dead Cell Removal Kit Miltenyi Biotec, Bergisch Gladbach, Germany 130-090-101 Chromium Next GEM Single Cell 3′ Kit v3.1, 4 rxns 10x Genomics, CA, USA PN-1000269 Chromium Next GEM Chip G Single Cell Kit, 16 rxns 10x Genomics PN-1000127 Dual Index Kit TT Set A, 96 rxns 10x Genomics PN-1000215 High Sensitivity DNA Kit Agilent Technology 5067-4727 Tissue-Tek® O.C.T. .. Compound Sakura Finetek Japan Co., Ltd., Tokyo, Japan 4583 13C5 L-glutamine Fujifilm Wako Pure Chemical Industries, Osaka, Japan CLM-1822-H-0.1 erythritol Tokyo Chemical Industry Co., Ltd., Tokyo, Japan E0021 Ultracentrifugation for 5 kDa cut-off filter Human Metabolome Technology, Tsuruoka, Japan UFC3LCCNB_HMT 96 well cell culture plates Greiner Bio-One 655180 Cell Counting Kit-8 Dojindo 343-07623 Eukitt ORSAtec, Bobingen, Germany 6.00.01.0001.06.01.EN BRANSON Sonifier 450 Branson Ultrasonics Corporation, CT, USA Instruments Product Name Supplier Catalog number Image Quant LAS 4000 mini luminescent image analyzer Cytiva, MA, USA NanoZoomer 2.0-RS Hamamatsu Photonics, Hamamatsu, Japan CellDrop BF DeNovix, DE, USA Seahorse XFp Analyzer Agilent Technology EVOS FL Thermo Fisher Scientific Qubit Invitrogen, MA, USA TapeStation Agilent Technology StepOne Real-time PCR system Thermo Fisher Scientific BX-41 Olympus, Tokyo, Japan Chromium controller 10x Genomics MTP-320 Corona Electric Co., Ltd., Ibaraki, Japan Tissue-Tek VIP5 Jr. Sakura Finetek Japan Co., Ltd TABLE 2 (Continued) Frontiers in Veterinary Science 06 frontiersin.org with ice-cold PBS.



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